|Date of Web Publication||14-Nov-2013|
Source of Support: None, Conflict of Interest: None
|How to cite this article:|
. Oral Presentations. Emerg Sci 2013;2, Suppl S1:15-6
Anti-proliferative and pro-apoptotic potential of rhein evaluated on different cancer cell lines of human origin
Atheer A Al-Ftlawi 1,2 , Anees A Al-Ftlawi 2 , Md Zafaryab 1 , Md Irshad 1 , Zakia Kazim 1 , Irfan Ahmad 1 , Ayaz Ahmad 2 , Zaffar Iqbal 1 , M Moshahid A Rizvi 1
1 Department of Biosciences, Genome Biology Lab, Jamia Millia Islamia, New Delhi, 2 Department of Pharmacology, Institute of Pharmacy, NIMS, Jaipur, Rajasthan, India
Plant derived compounds of therapeutic importance have been found to be safely as compared to the synthetic chemotherapeutic options. Rhein is one of the compound reported as potent biological origin antioxidant. In the present study Rhein was evaluated for its antiproliferative and pro-apoptotic potential against various cancer cells of human origin. Growth inhibition was monitored through MTT and LDH assays and the result was found significantly (P < 0.005) effective in different type of cancer cell lines viz (SiHa) cervical cancer, (MCF-7) breast adenocarcinoma and (HepG2) hepatoma cells. Pro-apoptotic activity was evaluated by reverse transcriptase-PCR analysis for P53, Bcl2 and Bax genes. Our result shows that Rhein induced apoptosis through activation of p53 and Bax with decreased expression of Bcl-2 gene. Therefore, this study suggested the utility and importance of Rhein in cancer therapy as it demonstrated strong behaviour against the multiple and important human cancer cells.
Sabutoclax induced mitophagy abrogates oral squamous cell carcinomas
Santanu Maji, Sabindra K Samal, Laxmipriya Pattanaik, Rupesh Dash
Department of Biotechnology, Institute of Life Sciences, (An Autonomous Institution of Government of India), Bhubaneswar, Odisha, India
Oral squamous cell carcinomas (OSCC) is the most prevalent cancer in India with approximately 80,000 cases reported each year. Despite intensive investigation, OSCC remains a formidable clinical challenge resulting in high frequencies of morbidity and mortality. Reprogramming tumour cells to undergo programmed cell death (apoptosis) represents a promising and powerful strategy for treating both local and metastatic disease. For example, altered expression of Bcl-2 family proteins is a common phenomenon during OSCC. Earlier studies suggest that association of Bcl-2 overexpression in OSCC indicates a paradox, i.e. in some tumour tissues; it is associated with favourable outcome, whereas in others the reverse scenario is true. In contrast, Mcl-1 over expression in OSCC is predominantly associated with a less favourable outcome. So we hypothesize that targeting Mcl-1 could be a useful strategy for cancer specific therapy in OSCC.
Using a unique Mcl-1 specific small molecule inhibitor BI-97C1 (Sabutoclax) we found that inhibition of Mcl-1 sensitized human OSCC cell lines to death depending upon their Mcl-1 expression level in cancer specific manner. In our study we found that Sabutoclax induces both the apoptosis and autophagy in OSCC cell lines. In autophagy microarray, we found that Sabutoclax significantly up-regulates Bnip3 (a member of BH3-only subset of the Bcl-2 family) in human SCC cells. Bnip3 is a hypoxia inducible factor-1 (HIF-1a) inducible gene that plays a major role in Mitophagy (a selective autophagy process that specifically degrade mitochondria). We assume that in OSCC, Sabutoclax induced mitophagy could abrogate OSCC tumour progression.
Post translational modifications of estrogen related receptor b regulates the transcriptional activity of its target genes
B Madhu Krishna, Sanjib Chaudhary, Sandip K Mishra
Cancer Biology Lab, Institute of Life Sciences, Nalco Square, Bhubaneswar, Odisha, India
Estrogen related receptors are a group of receptors belong to orphan nuclear receptor family. Estrogen related receptors share a sequence homology within the family members. As ERRb shares high sequence homology with ERs and the post translational modifications of ERs modulates the transcriptional activity of its target genes, we hypothesize that post translational modifications of ERRb may modulate the transcriptional activity of same or different ER target genes. Our present study shows that ERRb protein gets acetylated. Upon over expression of ERRb, transcriptional activity of Sirt1 promoter was enhanced. Bio-informatics analysis of sirt1 promoter revealed the presence of putative ERRE sites which was further confirmed by in vivo binding assay. It concludes post translational modifications of ERRb may regulate sirt1 contributing to cell cycle proliferation and breast cancer.
Design of CMOS circuit system in biosensor for early stage detection of cancer
Rajesh Laik, VijayNath, RN Gupta
Department of Biotechnology, Birla Institute of Technology, Mesra, Ranchi, Jharkand, India
CMOS low power SCC system for early detection of cancer is developed in the form of ASIC device, the signals are in the form of combination of NAND-NOR-NOT logic which senses the input biological system and converts the energy into voltage, with different approach of transduction, the mechanical is converted to wt/Hb, the photo system is changing into image, piezo electrical resistor system into voltage, the electrochemical P H system into ions. The workstation of bioinformatics with specific protein modeling approach extracts the database of the protein structure of the homologous organs of the animals and humans. Here, it has been shown all the proposed approaches for early stage detection of cancer, among these the best possible solution which can or will work best for early stage detection of cancer, is status of cancer progress with change in P H with its color pattern simultaneously the fooding pattern also shifts drastically from normal to cancer patients hence, with the behavioral aspects and looking at the patient and normal person we can easily make out the cancer patient. The fooding pattern in India changes from place to place, as per the temperature, so the variation in P H also changes, and due to wrong our system cycle and the internal clock also changes. Therefore we need to monitor the behavioral switching of our internal clock, and matches with the external CMOS driving circuitcan detect the error in the metabolic clock, the cell cycle pattern will indicate the disorder of the cell.So the P H based reference electrochemical system measures the status of cancer with time, the enzyme activity also changes.This alarms us to select the right food at regular time interval, the P H is a sensitive paper which absorbs moisture, VOCS, it very well monitors our digestive system starts from salivary amylase to pancreas. This gives the indication of free energy available, to perform any work, the wt/Hb indicator gives the detection of blood cancer risk, pressure system indicator measured by stethoscope, mercury of our lungs status, this alerts us to take fresh air, do breathing exrecise and pranayam to normalise our breathing pattern. Hence the CMOS driving circuit system does the important work to convert the P H into mVolt, the deflection of the nanomechanical cantilever sytem of P H variation is the proposed detection device, to be fabricated. The mathematical equation is derived here to calculate the concentration of hydrogen ion, free energy behavior of the cancer cell. 2/10 patients detected with cancer at cancer hospital due to exposed work at coal mining station, the patients professions are driller. They are the victims of mouth, oesopahgus cancer, and has become malignant the cancer has spreaded to back bone, the patients behaviour shows that he cannot lie down on the bed, or sit properly. The patient is under treatment at Apollo Cancer Research Centre IRWA. The image was captured by CMOS imaging system.
Steps in Cancer monitoring
- Health alert (a) Fooding pattern (b) Management of health care (c) precautionary measures
- Occupational health safety
- Internal timer to control cell cycle
- Time taken for repairing system to heal the cell
- Monitoring the salivary secretion system of our digestive system.
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